PRL-3/PTP4A3 Antibody (334402)

R&D Systems | Catalog # MAB3219

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Zebrafish

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 334402
View all PRL-3/PTP4A3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365

Specificity

Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PRL-3/PTP4A3 Antibody (334402)

Detection of Human/Mouse/Rat PRL-3 antibody by Western Blot.

Detection of Human/Mouse/Rat PRL‑3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PRL-3/PTP4A3 Antibody (334402)

Application
Recommended Usage

Western Blot

0.3 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line

Reviewed Applications

Read 3 reviews rated 3.7 using MAB3219 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRL-3/PTP4A3

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Long Name

Phosphatase of Regenerating Liver 3

Alternate Names

PRL3, PTP4A3

Entrez Gene IDs

11156 (Human); 19245 (Mouse)

Gene Symbol

PTP4A3

UniProt

O75365

Additional PRL-3/PTP4A3 Products

Product Documents for PRL-3/PTP4A3 Antibody (334402)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PRL-3/PTP4A3 Antibody (334402)

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for PRL-3/PTP4A3 Antibody (334402)

Customer Reviews for PRL-3/PTP4A3 Antibody (334402) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat PRL-3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    PRL-3/PTP4A3 Antibody (334402) MAB3219
  • Human/Mouse/Rat PRL-3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019
  • Human/Mouse/Rat PRL-3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018

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