PTK7/CCK4 Antibody

R&D Systems | Catalog # AF4499

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PTK7/CCK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308

Specificity

Detects human, mouse and rat PTK7/CCK4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PTK7/CCK4 Antibody

Detection of Human, Mouse, and Rat PTK7/CCK4 antibody by Western Blot.

Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human PTK7/CCK4 antibody by Simple WesternTM.

Detection of Human PTK7/CCK4 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of PTK7/CCK4 by Western Blot

Detection of PTK7/CCK4 by Western Blot

Validation of TIMP-3/HEK cell membrane proteome analysis by immunoblotting. Immunoblots (A) and their respective quantification (B) showing levels of PTK7, APP, ADAM15, CADM1, SDC4, and EphA4 in membrane proteins isolated from TIMP-3/HEK and control HEK 293 cells, (densitometric quantifications shown as mean values ± standard deviation; * p < 0.05, ** p < 0.01, Student’s t-test; from 3 to 6 separate experiments have been performed and analyzed). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33673623), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PTK7/CCK4 by Western Blot

Detection of PTK7/CCK4 by Western Blot

Validation of metalloprotease-dependent shedding of TIMP-3 regulated proteins by immunoblotting. Immunoblots (A) and their respective quantification (B, conditioned media; C, cell lysates) showing levels of PTK7, APP, CADM1, and ADAM15 in conditioned media or lysates of HEK 293 cells treated with or without marimastat (control). GAPDH was used as a loading control. Densitometric quantifications shown as mean values ± standard deviation; *** p < 0.005, Student’s t-test; (n = 6). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33673623), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PTK7/CCK4 by Western Blot

Detection of PTK7/CCK4 by Western Blot

Analysis of protein changes by immunoblotting. (A) Immunoblots showing protein abundance of TIMP-3, PTK7, APP, ADAM15, and CADM1 in the conditioned media of TIMP-3/HEK and control HEK 293 cells, as well as levels of PTK7, APP, ADAM15, CADM1 and EphA4 in the cell lysates. Calnexin and GAPDH were used as loading controls. Densitometric quantifications of specific proteins in the conditioned media (B) and cell lysates (C) are displayed as mean values ± standard deviation (** p < 0.01, *** p < 0.005, Student’s t-test; from 3 to 6 separate experiments have been performed and analyzed). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33673623), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for PTK7/CCK4 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line

Reviewed Applications

Read 1 review rated 5 using AF4499 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTK7/CCK4

CCK4 (colon carcinoma kinase 4, also known as PTK7) is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, CCK4 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner.

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Entrez Gene IDs

5754 (Human); 71461 (Mouse); 301242 (Rat)

Gene Symbol

PTK7

UniProt

Q13308

Additional PTK7/CCK4 Products

Product Documents for PTK7/CCK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PTK7/CCK4 Antibody

For research use only

Citations for PTK7/CCK4 Antibody

Customer Reviews for PTK7/CCK4 Antibody (1)

5 out of 5
1 Customer Rating
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4 Stars
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1 Stars
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23095747
    Species: Human
    Verified Customer | Posted 01/08/2015

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