RalA Antibody (399527)

R&D Systems | Catalog # MAB3270

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 399527
View all RalA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233

Specificity

Detects human, mouse, and rat RalA in Western blots. In Western blots, no cross-reactivity with recombinant human RalB is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for RalA Antibody (399527)

Detection of Human/Mouse/Rat RalA antibody by Western Blot.

Detection of Human/Mouse/Rat RalA by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat RalA Monoclonal Antibody (Catalog # MAB3270) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (2 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for RalA Antibody (399527)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein A

Alternate Names

Rasl1

Entrez Gene IDs

5898 (Human); 56044 (Mouse); 81757 (Rat)

Gene Symbol

RALA

UniProt

P11233

Additional RalA Products

Product Documents for RalA Antibody (399527)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for RalA Antibody (399527)

For research use only

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