The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 399527
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Product Specifications
Immunogen
E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233
Met1-Leu206
Accession # P11233
Specificity
Detects human, mouse, and rat RalA in Western blots. In Western blots, no cross-reactivity with recombinant human RalB is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for RalA Antibody (399527)
Detection of Human/Mouse/Rat RalA by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat RalA Monoclonal Antibody (Catalog # MAB3270) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (2 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for RalA Antibody (399527)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RalA
Long Name
Ras-like Protein A
Alternate Names
Rasl1
Gene Symbol
RALA
UniProt
Additional RalA Products
Product Documents for RalA Antibody (399527)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for RalA Antibody (399527)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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