Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, and modulates the activation of several signal transduction cascades. By binding the MAP3K Raf-1, unphosphorylated RKIP inhibits activation of the MAP2K MEK. PKC-phosphorylated RKIP dissociates from Raf‑1 and inhibits GRK2, resulting in sustained G-protein signaling. Expressed in many tissues, RKIP is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human RKIP
Met1-Lys187
Accession # P30086
Met1-Lys187
Accession # P30086
Specificity
Detects endogenous human, mouse, and rat RKIP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RKIP Antibody
Detection of Human/Mouse/Rat RKIP by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for RKIP Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line
Sample: HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line
Reviewed Applications
Read 4 reviews rated 3.8 using AF4538 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RKIP
Long Name
Raf kinase inhibitory protein
Alternate Names
HCNPpp, Neuropolypeptide h3, PBP, PEBP-1, PEBP1
Gene Symbol
PEBP1
UniProt
Additional RKIP Products
Product Documents for RKIP Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for RKIP Antibody
For research use only
Related Research Areas
Customer Reviews for RKIP Antibody (4)
3.8 out of 5
4 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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