SF20/MYDGF Antibody (2562A)

R&D Systems | Catalog # MAB1104

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2562A
View all SF20/MYDGF Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived mouse SF20/MYDGF
Val25-Leu166
Accession # Q9CPT4

Specificity

Detects mouse SF20/MYDGF in direct ELISAs. Detects human, mouse, and rat SF20/MYDGF in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF20/MYDGF Antibody (2562A)

Detection of Human, Mouse, and Rat SF20/MYDGF antibody by Western Blot.

Detection of Human, Mouse, and Rat SF20/MYDGF by Western Blot.

Western blot shows lysates of HT-2 mouse T cell line, NIH-3T3 mouse embryonic fibroblast cell line, DU145 human prostate carcinoma cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SF20/MYDGF on Tramp-C1 Mouse Cell Line antibody by Flow Cytometry.

Detection of SF20/MYDGF on Tramp-C1 Mouse Cell Line by Flow Cytometry.

Tramp-C1 mouse cell line was stained with Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104, filled histogram) or Rabbit IgG isotype control antibody (Catalog # MAB1050, open histogram) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
SF20/MYDGF antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC).

SF20/MYDGF in RAW 264.7 Mouse Cell Line.

SF20/MYDGF was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SF20/MYDGF antibody in Human Liver by Immunohistochemistry (IHC-P).

SF20/MYDGF in Human Liver.

SF20/MYDGF was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse and Rat SF20/MYDGF antibody by Simple WesternTM.

Detection of Mouse and Rat SF20/MYDGF by Simple WesternTM.

Simple Western lane view shows lysates of HT‑2 mouse T cell line and Rat‑2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat SF20/MYDGF Monoclonal Antibody (Catalog # MAB1104). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for SF20/MYDGF Antibody (2562A)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Immunohistochemistry

0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Mouse Tramp-C1 cell line

Simple Western

20 µg/mL
Sample: HT‑2 mouse T cell line and Rat‑2 rat embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: HT‑2 mouse T cell line, NIH‑3T3 mouse embryonic fibroblast cell line, DU145 human prostate carcinoma cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB1104 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

References

  1. Korf-Klingebiel, M. et al. (2015) Nat. Med. 10:3778.
  2. Wang, P. et al. (2004) Cell. Mol. Life Sci. 61:2405.
  3. Sunagozaka, H. et al. (2011) Int. J. Cancer 129:1576.
  4. Bortnov, V. et al. (2018) J. Biol. Chem. 293:13166.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human); 28106 (Mouse)

Gene Symbol

MYDGF

UniProt

Q9CPT4

Additional SF20/MYDGF Products

Product Documents for SF20/MYDGF Antibody (2562A)

Certificate of Analysis

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Product Specific Notices for SF20/MYDGF Antibody (2562A)

For research use only

Citations for SF20/MYDGF Antibody (2562A)

Customer Reviews for SF20/MYDGF Antibody (2562A) (1)

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  • Human/Mouse/Rat SF20/MYDGF Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Eosinophils
    Species: Mouse
    Verified Customer | Posted 10/21/2021
    SF20/MYDGF Antibody (2562A) MAB1104

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Protocols

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