Slit3 Antibody

Slit3 in 13 D.P.C. Mouse Embryo.

Slit3 was detected in immersion fixed paraffin-embedded sections of 13 d.p.c. mouse embryo using Goat Anti-Human/Mouse/Rat Slit3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3629) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing braiin and cartillage.

Detection of Slit3 by Western Blot

VDC stimulates the expression of osteoblast‐specific proteins by the Wnt/SHN3 pathway. A) Representative images and corresponding quantitative analysis of immunofluorescence staining results of OCN and beta ‐catenin treated with 1, 5, and 10 µm VDC for 5 days (n = 5). Scale bar, 50 µm. B) Representative images and quantitative analysis of immunofluorescence staining of OCN and beta ‐catenin treated with VDC (5 µm) for 3 days, 5 days, and 8 days (n = 5). Scale bar, 50 µm. C) Representative images of western blot showing the expression levels of beta ‐catenin, NF‐ kappa B, GSK‐3 beta, phosphorylated (p)‐GSK‐3 beta, ERK1/2, p‐ERK1/2, Slit3 and normalized to the expression of GAPDH. Under induction conditions (50 µg mL−1 ascorbic acid and 5 mm beta ‐glycerophosphate), MC3T3‐E1 cells were cultured with VDC (1, 5, 10 µm) or 0.1% DMSO (control group) for 5 days. The solvent control group (Control) was treated with 0.1% DMSO (n = 3). D) Quantitative analysis of the relative protein expression for beta ‐catenin, NF‐ kappa B, p‐GSK‐3 beta and p‐ERK1/2 and Slit3. Data represent mean ± SD, ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus the Control group by one‐way ANOVA with Tukey's post‐hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40192033), licensed under a CC-BY license. Not internally tested by R&D Systems.