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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 705111
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Product Specifications
Immunogen
E. coli-derived recombinant human STIM1
Leu23-Thr182
Accession # Q13586
Leu23-Thr182
Accession # Q13586
Specificity
Detects human STIM1 in direct ELISAs and Western blots.
In direct ELISAs and Western blots, no
cross-reactivity with recombinant human STIM2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for STIM1 Antibody (705111)
Detection of Human, Mouse, and Rat STIM1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat STIM1 Monoclonal Antibody (Catalog # MAB6976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STIM1 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human STIM1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STIM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat STIM1 Monoclonal Antibody (Catalog # MAB6976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STIM1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for STIM1 Antibody (705111)
Application
Recommended Usage
Knockout Validated
STIM1
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
STIM1 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: STIM1
Long Name
Stromal Interaction Molecule 1
Alternate Names
GOK
Gene Symbol
STIM1
UniProt
Additional STIM1 Products
Product Documents for STIM1 Antibody (705111)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for STIM1 Antibody (705111)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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