Substance P Antibody (266815)

R&D Systems | Catalog # MAB4375

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 266815
View all Substance P Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled mouse Substance P synthetic peptide
RPKPQQFFGLM

Specificity

Detects human, mouse, and rat Substance P.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Substance P Antibody (266815)

Substance P antibody in Human Spinal Cord by Immunohistochemistry (IHC-P).

Substance P in Human Spinal Cord.

Substance P was detected in immersion fixed paraffin-embedded sections of human spinal cord using 8 µg/mL Human/Mouse/Rat Substance P Monoclonal Antibody (Catalog # MAB4375) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Substance P Antibody (266815)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord

Reviewed Applications

Read 3 reviews rated 4.7 using MAB4375 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Substance P

Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.

Alternate Names

Neurokinin-1, TAC1

Entrez Gene IDs

6863 (Human)

Gene Symbol

TAC1

Additional Substance P Products

Product Documents for Substance P Antibody (266815)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Substance P Antibody (266815)

For research use only

Citations for Substance P Antibody (266815)

Customer Reviews for Substance P Antibody (266815) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat Substance P Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Dorsal root ganglion
    Species: Mouse
    Verified Customer | Posted 10/20/2021
    Substance P Antibody (266815) MAB4375
  • Human/Mouse/Rat Substance P Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spinal cord
    Species: Mouse
    Verified Customer | Posted 09/02/2021
    Substance P Antibody (266815) MAB4375
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23232501
    Species: Mouse
    Verified Customer | Posted 02/20/2015

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Protocols

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FAQs

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