Syntaxin 7 Antibody

R&D Systems | Catalog # AF5478

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Syntaxin 7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 7
Asn21-Glu187
Accession # O15400

Specificity

Detects human, mouse, and rat Syntaxin 7 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Syntaxin 7 Antibody

Detection of Human Syntaxin 7 antibody by Western Blot.

Detection of Human Syntaxin 7 by Western Blot.

Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, and Rat Syntaxin 7 antibody by Western Blot.

Detection of Human, Mouse, and Rat Syntaxin 7 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, BaF3 mouse pro-B cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 7 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Syntaxin 7 in HeLa Human Cell Line.

Syntaxin 7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Syntaxin 7 in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells.

Syntaxin 7 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Syntaxin 7 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa Human Cervical Epithelial Carcinoma, Daudi Human Burkitt's Lymphoma (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia (Negative) Cell Line Cells.

Western Blot

1 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, BaF3 mouse pro-B cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 3 reviews rated 4 using AF5478 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin 7

Syntaxin 7 (STX7) is a widely expressed protein embedded in endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX7 is involved in endocytic trafficking from early endosomes to late endosomes and lysosomes. This is in contrast to STX8, which is involved in clathrin-independent vesicular transport. Human STX7 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 261 amino acids (aa) in length. It contains a coiled-coil region (aa 47‑69), a t-SNARE domain (aa 165‑227) that is likely involved in protein-protein interactions, and a short, two amino acid, C-terminal luminal sequence. Over aa 21‑187, human STX7 shares 97% aa identity with mouse STX7.

Alternate Names

STX7

Entrez Gene IDs

8417 (Human); 53331 (Mouse); 60466 (Rat)

Gene Symbol

STX7

UniProt

O15400 (Human)

Additional Syntaxin 7 Products

Product Documents for Syntaxin 7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Syntaxin 7 Antibody

For research use only

Citations for Syntaxin 7 Antibody

Customer Reviews for Syntaxin 7 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat Syntaxin 7 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Syntaxin 7 Antibody AF5478
  • Human/Mouse/Rat Syntaxin 7 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018
  • Human/Mouse/Rat Syntaxin 7 Antibody
    Name: Scott Messenger
    Application: Western Blot
    Sample Tested: T84 human colon carcinoma cell line
    Species: Human
    Verified Customer | Posted 04/25/2017
    whole cell lystae 100 ug protein 10% gel some non-specific bands
    Syntaxin 7 Antibody AF5478

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Protocols

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