Teneurin-4 Antibody

R&D Systems | Catalog # AF6320

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Teneurin-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Teneurin-4
Lys61-Lys340
Accession # Q6N022

Specificity

Detects human, mouse, and rat Teneurin-4 in Western blots and human Teneurin-4 in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Teneurin-1 is observed, and less than 1% cross-reactivity with rhTeneurin-2 and rhTeneurin-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Teneurin-4 Antibody

Detection of Human and Mouse Teneurin‑4 by Western Blot.

Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and L‑929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin‑4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Rat Teneurin‑4 by Western Blot.

Western blot shows lysates of Rat cortical stem cell. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6320) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Teneurin‑4 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Teneurin-4 by Western Blot

Detection of Teneurin-4 by Western Blot

The effect of fraction ACH3 on neurite outgrowth (A). MTT assay was performed to clarify the effect of fraction ACH3 on cell viability (B). GAP-43 and Teneurin4 expression shown in a representative Western blot (C). Normalized values of both GAP-43 and Teneurin-4 against beta -actin (D). All data are presented as the mean ± SEM, * p < 0.05 vs. control; +p < 0.05 vs. 10 μM RA; #p < 0.05 vs. high glucose-treated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36079924), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Teneurin-4 by Western Blot

Detection of Teneurin-4 by Western Blot

The effect of fraction ACH3 on neurite outgrowth (A). MTT assay was performed to clarify the effect of fraction ACH3 on cell viability (B). GAP-43 and Teneurin4 expression shown in a representative Western blot (C). Normalized values of both GAP-43 and Teneurin-4 against beta -actin (D). All data are presented as the mean ± SEM, * p < 0.05 vs. control; +p < 0.05 vs. 10 μM RA; #p < 0.05 vs. high glucose-treated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36079924), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Teneurin-4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, L‑929 mouse fibroblast cell line, and Rat cortical stem cell

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Teneurin-4

Teneurin-4 (also Ten-m4, Doc4 and tenascin-M4) is a 250-300 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is widely expressed in the embryo, and in adult, participates in cell-to-cell adhesion, and may communicate ER stress levels. Human Teneurin-4 is a 2769 amino acid (aa) type II TM glycoprotein. It contains a 345 aa cytoplasmic region (aa 1-345), plus a 2403 aa extracellular domain (ECD) (aa 367‑2769). The ECD possesses eight sequential EGF-like domains (aa 561-831), five NHL repeats, each of which form a beta -propeller (aa 1216-1566), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. C-terminal cleavage generates a short 5 kDa, 41 aa peptide (aa 2726-2766) termed TCAP-1 that shows bioactivity. Teneurin-4 is hypothesized to form heterodimers with other teneurins. Over aa 61-340, human Teneurin-4 shares 95% aa identity with mouse Teneurin-4.

Alternate Names

ODZ4, Ten-M4, Tenascin-M4, Teneurin4, TNM4

Entrez Gene IDs

26011 (Human)

Gene Symbol

TENM4

UniProt

Q6N022 (Human)

Additional Teneurin-4 Products

Product Documents for Teneurin-4 Antibody

Certificate of Analysis

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Product Specific Notices for Teneurin-4 Antibody

For research use only

Citations for Teneurin-4 Antibody

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