YAP1 Antibody (867711)

R&D Systems | Catalog # MAB8094

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western, CyTOF-reported

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 867711
View all YAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human YAP1
Ala347-Leu504
Accession # P46937

Specificity

Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for YAP1 Antibody (867711)

Detection of Human, Mouse, and Rat YAP1 antibody by Western Blot.

Detection of Human, Mouse, and Rat YAP1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YAP1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
YAP1 antibody in Human Prostate by Immunohistochemistry (IHC-P).

YAP1 in Human Prostate.

YAP1 was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # MAB8094) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human YAP1 antibody by Simple WesternTM.

Detection of Human YAP1 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for YAP1 at approximately 79 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat YAP1 Monoclonal Antibody (Catalog # MAB8094). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human YAP1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human YAP1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat YAP1 Monoclonal Antibody (Catalog # MAB8094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for YAP1 Antibody (867711)

Application
Recommended Usage

CyTOF-reported

Brodie, T.M. et al. (2018) Cytometry Part A. 93: 406. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human prostate

Knockout Validated

YAP1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in YAP1 knockout HeLa cell line.

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line

Reviewed Applications

Read 1 review rated 5 using MAB8094 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YAP1

YAP1 (Yes-associated protein 1), also called YAP65 or Yorkie Homolog, is a widely expressed 65 kDa cytoplasmic and nuclear transcriptional regulator protein in the Hippo pathway that regulates organ size. The 504 amino acid (aa) human YAP1 contains two WW (tryptophan-containing protein binding) domains (aa 171-263), a transactivation domain (aa 219-504) and 19 sites that may be phosphorylated by kinases such as LATS1 and 2, MAPK8 and 9, CK1, or ABL1. Isoforms of 488, 454 and 326 aa lack aa 328-343, 230-267 (plus a 3 aa substitution for aa 329-343), or 1-178, respectively. YAP1 binds the Yes tyrosine kinase SH3 domain and many other proteins, notably ErbB4. YAP1 expression is increased in some liver and prostate cancers. Within aa 347-504, human YAP1 shares 94% and 93% aa sequence identity with mouse and rat YAP1, respectively.

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Entrez Gene IDs

10413 (Human); 22601 (Mouse)

Gene Symbol

YAP1

UniProt

P46937

Additional YAP1 Products

Product Documents for YAP1 Antibody (867711)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for YAP1 Antibody (867711)

For research use only

Citations for YAP1 Antibody (867711)

Customer Reviews for YAP1 Antibody (867711) (1)

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  • Human/Mouse/Rat YAP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 10/21/2021
    YAP1 Antibody (867711) MAB8094

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