RNA Polymerase II/POLR2A Antibody

R&D Systems | Catalog # AF6160

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Control

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RNA Polymerase II/POLR2A
Leu15-Leu354
Accession # P24928

Specificity

Detects human and mouse RNA Polymerase II/POLR2A in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A Antibody

Detection of Human and Mouse RNA Polymerase II/-POLR2A antibody by Western Blot.

Detection of Human and Mouse RNA Polymerase II/-POLR2A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and HT-2 mouse T cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse RNA Polymerase II/-POLR2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for RNA Polymerase II/-POLR2A at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for RNA Polymerase II/POLR2A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, and HT‑2 mouse T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RNA Polymerase II/POLR2A

POLR2A (DNA-directed RNA Polymerase II subunit RPB1; also subunit A) is a 200‑220 kDa member of the RNA polymerase beta -chain family of proteins. It is ubiquitously expressed and represents the largest subunit of RNA polymerase II, the catalytic mechanism for the synthesis of mRNA. Human POLR2A is 1970 amino acids (aa) in length. It contains an N-terminus with six RNA polymerase domains (aa 15‑1079), and a C-terminus (CTD) with 52 seven aa repeats (‑YSPTSPS‑) that serve as a platform for polymerase subunit interaction. When these sequences are hyperphosphorylated, this subunit is called RNA pol IIo; when hypophosphorylated, the subunit becomes RNA pol IIa. Isoforms exist that show a 60 aa substitution for aa 1‑95, a 24 aa substitution for aa 1‑445, and an Arg substitution for aa 1498‑1536. Full-length human and mouse POLR2A sequences differ by only one amino acid (Thr1856Ala).

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Entrez Gene IDs

5430 (Human); 20020 (Mouse); 363633 (Rat)

Gene Symbol

POLR2A

UniProt

P24928 (Human)

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A Antibody

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A Antibody

For research use only

Related Research Areas

Epigenetics

Citations for RNA Polymerase II/POLR2A Antibody

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