TANK Antibody

R&D Systems | Catalog # AF4755

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TANK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TANK
Ala157-Thr448
Accession # P70347

Specificity

Detects endogenous human and mouse TANK in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TANK Antibody

Detection of Human/Mouse TANK antibody by Western Blot.

Detection of Human/Mouse TANK by Western Blot.

Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse TANK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TANK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for TANK Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TANK

TRAF (tumor necrosis factor receptor-associated factor) family member-associated NF-kappaB activator (TANK) maintains TRAF1, TRAF2 and TRAF3 proteins in their latent states (cytoplasmic sequestration). TANK binds to their TRAF-c domains inhibiting TRAF signaling and NF kappa B activation. TANK may function as an adaptor molecule in multiple antiviral pathways. Two transcript variants encoding different isoforms for this gene have been found.

Long Name

TRAF Family Member-Associated NFKB Activator

Alternate Names

I-TRAF

Entrez Gene IDs

10010 (Human); 21353 (Mouse); 252961 (Rat)

Gene Symbol

TANK

UniProt

P70347

Additional TANK Products

Product Documents for TANK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for TANK Antibody

For research use only

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FAQs

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Associated Pathways

Toll-Like Receptor Signaling Pathways
Toll-Like Receptor Signaling Pathway Thumbnail