TGF-beta RI/ALK-5 Antibody (141231)

R&D Systems | Catalog # MAB5871

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 141231
View all TGF-beta RI/ALK-5 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RI/ALK-5
Ala21-Glu121
Accession # BAA05023

Specificity

Detects human and mouse TGF-beta RI/ALK-5 in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta  RII, rhTGF‑ beta  RIIb, or rhTGF‑ beta  RIII.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for TGF-beta RI/ALK-5 Antibody (141231)

Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

Detection of Mouse TGF-beta RI/ALK-5 by Western Blot

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TGF-beta RI/ALK-5 by Flow Cytometry

Detection of Mouse TGF-beta RI/ALK-5 by Flow Cytometry

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF-beta RI/ALK-5 Antibody (141231)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TGF-beta  RI/ALK‑5 Fc Chimera (Catalog # 587-RI) and Recombinant Human TGF-beta  RI/ALK‑5 Fc Chimera (Catalog # 3025-BR)

Reviewed Applications

Read 3 reviews rated 3.7 using MAB5871 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References

  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

BAA05023

Additional TGF-beta RI/ALK-5 Products

Product Documents for TGF-beta RI/ALK-5 Antibody (141231)

Certificate of Analysis

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Product Specific Notices for TGF-beta RI/ALK-5 Antibody (141231)

For research use only

Citations for TGF-beta RI/ALK-5 Antibody (141231)

Customer Reviews for TGF-beta RI/ALK-5 Antibody (141231) (3)

3.7 out of 5
3 Customer Ratings
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse TGF-beta  RI/ALK-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Dermal fibroblasts
    Species: Human
    Verified Customer | Posted 07/17/2021
    TGF-beta RI/ALK-5 Antibody (141231) MAB5871
  • Human/Mouse TGF-beta  RI/ALK-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 06/29/2021
    TGF-beta RI/ALK-5 Antibody (141231) MAB5871
  • Human/Mouse TGF-beta  RI/ALK-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: colo357
    Species: Human
    Verified Customer | Posted 06/28/2016
    Bio-Techne Response
    R&D Systems Technical Service is investigating.

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