Wnt-8b Antibody

R&D Systems | Catalog # AF3367

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Wnt-8b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6

Specificity

Detects human and mouse Wnt-8b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Wnt-8a is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Wnt-8b Antibody

Detection of Human Wnt-8b antibody by Western Blot.

Detection of Human Wnt‑8b by Western Blot.

Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Wnt-8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3367) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-8b at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Wnt-8b Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NCI‑N87 human gastric carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Wnt-8b

Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Alternate Names

Wnt8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Gene Symbol

WNT8B

UniProt

Q9WUD6

Additional Wnt-8b Products

Product Documents for Wnt-8b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Wnt-8b Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Citations for Wnt-8b Antibody

Customer Reviews for Wnt-8b Antibody

There are currently no reviews for this product. Be the first to review Wnt-8b Antibody and earn rewards!

Have you used Wnt-8b Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies