YY1 Antibody (854847)

R&D Systems | Catalog # MAB3784

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 854847
View all YY1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human YY1
His75-Gln275
Accession # P25490

Specificity

Detects human YY1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Rex-1/ZFP42 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for YY1 Antibody (854847)

Detection of Human and Mouse YY1 antibody by Western Blot.

Detection of Human and Mouse YY1 by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for YY1 Antibody (854847)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YY1

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Entrez Gene IDs

7528 (Human); 22632 (Mouse); 24919 (Rat)

Gene Symbol

YY1

UniProt

P25490

Additional YY1 Products

Product Documents for YY1 Antibody (854847)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for YY1 Antibody (854847)

For research use only

Citations for YY1 Antibody (854847)

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