ZBTB7A/Pokemon Antibody (466407)

Detection of Human and Mouse ZBTB7A/Pokemon by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ZBTB7A/Pokemon Monoclonal Antibody (Catalog # MAB3496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZBTB7A/Pokemon at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Detection of ZBTB7A/Pokemon by Western Blot

Pomalidomide enhances fetal hemoglobin production induced by 3′HS-1 deletion.(A) qRT-PCR quantification of BCL11A in the pomalidomide-treated WT and 3′-HS-1-deleted HUDEP2 cell clones. (B) Composition of beta -like globin by qRT-PCR in clones treated with DMSO and 1 μM pomalidomide described in panel (A). (C) The flow cytometry plot of HbF in clones treated with DMSO and 1 μM pomalidomide described in panel (A). (D) Western blot of BCL11A, ZBTB7A, beta -globin, and gamma -globin clones treated with DMSO and 1 μM pomalidomide described in panel (A). (E) The differentiation stage of HUDEP-2 cell clones used in panel (A) profiled by flow cytometry of CD71 and CD235a.Figure 2—figure supplement 2—source data 1.The immunoblot data of BCL11A, ZBTB7A, beta -actin, beta -globin, and gamma -globin of clones displayed in Figure 2—figure supplement 2.The immunoblot data of BCL11A, ZBTB7A, beta -actin, beta -globin, and gamma -globin of clones displayed in Figure 2—figure supplement 2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34585664), licensed under a CC-BY license. Not internally tested by R&D Systems.