Human MSP/MST1 beta Chain Antibody (68801) [Biotin]
R&D Systems | Catalog # BAM3521
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2B Clone # 68801
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP beta chain
Accession # P26927
Accession # P26927
Specificity
Detects recombinant human (rh)MSP in direct ELISAs and Western blots. When used in combination with the monoclonal capture antibody (Catalog # MAB352) in sandwich ELISAs, less than 0.001% cross-reactivity was observed with rhHGF, rhIL-9 R, rhMSP alpha or rmMSP R.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MSP/MST1 beta Chain Antibody (68801) [Biotin]
Human MSP / MST1 ELISA Standard Curve
Recombinant Human MSP/MST1 (Catalog # 352-MS) was serially diluted and captured by Mouse Anti-Human MSP/MST1 alpha Chain Monoclonal Antibody (Catalog # MAB352) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MSP/MST1 beta Chain Monoclonal Antibody (Catalog # MAB735) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human MSP/MST1 beta Chain Antibody (68801) [Biotin]
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only
Sample: Recombinant Human MSP (Catalog # 352-MS)
under non-reducing conditions only
Human MSP Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MSP/MST1 beta Chain
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Gene Symbol
MST1
UniProt
Additional MSP/MST1 beta Chain Products
Product Documents for Human MSP/MST1 beta Chain Antibody (68801) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MSP/MST1 beta Chain Antibody (68801) [Biotin]
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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