Human MuSK Antibody

R&D Systems | Catalog # AF3904

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MuSK Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MuSK
Glu22-Ser503
Accession # O15146

Specificity

Detects human MuSK in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant rat MuSK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MuSK Antibody

Detection of Mouse MuSK by Western Blot

Detection of Mouse MuSK by Western Blot

Sorbs1 is enriched at AChR aggregates, and Sorbs1 RNAi blocks AChR clustering in vitro. (A) Treatment of myotubes with siRNA directed against Sorbs1 blocks AChR clustering in C2C12 cells. Montages containing sixteen fields at a magnification of 10× were analyzed with ImageJ software (NIH). (B) Sorbs1 siRNA significantly reduces Sorbs1 protein expression in myotubes. (C) Sorbs1 protein is highly enriched at sites where AChRs aggregate. (D) Agrin stimulates tyrosine phosphorylation of MuSK and Dok-7 at similar levels in myotubes treated with Sorbs1 siRNA. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26527617), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MuSK Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MuSK

Reviewed Applications

Read 2 reviews rated 3 using AF3904 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MuSK

MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22‑503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

UniProt

O15146

Additional MuSK Products

Product Documents for Human MuSK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MuSK Antibody

For research use only

Citations for Human MuSK Antibody

Customer Reviews for Human MuSK Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
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3 Stars
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2 Stars
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1 Stars
50%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human MuSK Antibody
    Name: Mia Huang
    Application: Simple Western
    Sample Tested: Differentiated C2C12 myoblast cells
    Species: Mouse
    Verified Customer | Posted 05/19/2017
    C2C12 differentiated myotubes, following IP with an anti-MuSK antibody (different from this Ab), SDS-PAGE, and transferred into PVDF membrane. After blocking with 5% BSA/TBST, blot was probed with 1:400 of AF562 (4C, o/n). Subsequent probing with a HRP-anti-goat secondary Ab yielded excellent publishable results, with 100 kDa MuSK band visible.
    Human MuSK Antibody AF3904
  • Human MuSK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Differentiated C2C12 myoblast cells
    Species: Mouse
    Verified Customer | Posted 05/16/2017
    Differentiated C2C12 mouse skeletal muscle lysates IP'ed for MuSK using a donated Ab. IP works well and I can detect phosphorylation of MuSK using a general phosphotyrosine Ab, but upon stripping, blocking, and probing with this Ab, I am unable to detect MuSK (perhaps due to high background signal). Probing with a different MuSK Ab is much cleaner
    Human MuSK Antibody AF3904
    Bio-Techne Response
    Technical Service is following up

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