Human NEDD9/CASL Antibody (584825)

R&D Systems | Catalog # MAB5459

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 584825
View all NEDD9/CASL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NEDD9/CASL
Lys153-Asn366
Accession # Q14511

Specificity

Detects human NEDD9/CASL in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NEDD4 or rhNEDD8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NEDD9/CASL Antibody (584825)

Detection of Human NEDD9/CASL antibody by Western Blot.

Detection of Human NEDD9/CASL by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human NEDD9/CASL Monoclonal Antibody (Catalog # MAB5459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for NEDD9/CASL at approximately 110 kDa & 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human NEDD9/CASL Antibody (584825)

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD9/CASL

NEDD9 (also HEF1 and CASL) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Tyr-rich SH2-binding region (aa 90-350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710-760). NEDD9 usually runs at 105 or 115 kDa in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155-188, while two others show 20 or 7 aa substitutions for aa 155-834 or 1-153, respectively. Over aa 153-366, human and mouse NEDD9 share 85% aa identity.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9

Alternate Names

CAS2, CASL, HEF1, p105

Entrez Gene IDs

4739 (Human); 18003 (Mouse); 291044 (Rat)

Gene Symbol

NEDD9

UniProt

Q14511 (Human)

Additional NEDD9/CASL Products

Product Documents for Human NEDD9/CASL Antibody (584825)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NEDD9/CASL Antibody (584825)

For research use only

Related Research Areas

Integrins and Associated Molecules

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