Human Neurogenin-3 Antibody

Detection of Human Neurogenin‑3 by Western Blot.

Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin-3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Neurogenin‑3 in Human Placenta.

Neurogenin‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell nuclei in decidual cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Neurogenin-3 by Immunocytochemistry/ Immunofluorescence

Immunofluorescent staining confirms the high frequency of NEUROG3 expression within epsilon cells. (A, C, D) All images shown are a single confocal Z-section. (A) Representative images showing the positive nuclear labeling of NEUROG3 in epsilon cells (left panel), other endocrine cells (middle panel) and exocrine cells (right panel) in postnatal human pancreatic tissue sections. Zoomed-in views of boxed regions are shown at the bottom of each panel. Color channels are: DAPI, blue; GHRL, green; INS+GCG+SST (a combination of three antibodies against individual proteins), yellow; NEUROG3, red. (B) Quantifications of NEUROG3+ cells in different populations. All cells from each section of the donor tissues were counted. Each data point represents aggregated results from one donor. “Other endocrine” corresponds to beta (INS+) or alpha (GCG+) or delta (SST+) cells. (C, D) Co-labeling of GHRL, NEUROG3 and Ki67. (C) The majority of NEUROG3+ cells are not proliferating. (D) in rare instances, coexpression of Ki67 and NEUROG3 can be observed. Color channels are: DAPI, blue; GHRL, green; Ki67, white; NEUROG3, red. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34566896), licensed under a CC-BY license. Not internally tested by R&D Systems.