Human Neuroplastin Isoform 1 Antibody

R&D Systems | Catalog # AF5174

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Neuroplastin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 55 isoform 1
Gln29-Ser219
Accession # Q9Y639.1

Specificity

Detects human Neuroplastin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Neuroplastin Isoform 1 Antibody

Detection of Human Neuroplastin antibody by Western Blot.

Detection of Human Neuroplastin by Western Blot.

Western blot shows lysates of human brain (cerebellum, hypothalamus, and hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neuroplastin antibody in Human Brain by Immunohistochemistry (IHC-P).

Neuroplastin in Human Brain.

Neuroplastin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 1.7 µg/mL Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the dendrites of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Neuroplastin Isoform 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

0.2 µg/mL
Sample: Human brain (cerebellum, hypothalamus, and hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuroplastin

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52‑57 kDa member of the Ig-superfamily. It is widely expressed, and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32‑119 and 122‑213) and a 38 aa cytoplasmic region (aa 245‑282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N-terminal 116 aa Ig-like domain-containing insert after Asn30, and interacts homophilically. There is a third potential splice variant that shows an alternate start site at Met85 accompanied by a 23 aa substitution for aa 200‑282. Over aa 29‑229, human Neuroplastin is 91% aa identical to mouse Neuroplastin.

Alternate Names

AW554172, GP55, GP66, NPTN, SDFR1, SDR1

Entrez Gene IDs

27020 (Human); 20320 (Mouse); 56064 (Rat)

Gene Symbol

NPTN

UniProt

Q9Y639.1

Additional Neuroplastin Products

Product Documents for Human Neuroplastin Isoform 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Neuroplastin Isoform 1 Antibody

For research use only

Citations for Human Neuroplastin Isoform 1 Antibody

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Protocols

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