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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Accession # P12036
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human NF-H Antibody (1016103)
Detection of Human NF‑H by Western Blot.
Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF‑H at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human NF‑H by Simple WesternTM.
Simple Western shows lysates of human motor cortex and human hippocampus, loaded at 0.2 mg/ml. A specific band was detected for NF‑H at approximately 270-350 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human NF-H Antibody (1016103)
Simple Western
Sample: Human motor cortex and human hippocampus
Western Blot
Sample: IMR‑32 human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: NF-H
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional NF-H Products
Product Documents for Human NF-H Antibody (1016103)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human NF-H Antibody (1016103)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars