Human Nicastrin Antibody (716910)

Nicastrin (NCT) is a 150‑160 kDa member of the nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as notch-1 and APP. Mature human nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.