Human Nidogen-1/Entactin Antibody (302117)

R&D Systems | Catalog # MAB2570

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 302117
View all Nidogen-1/Entactin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin
Leu29-Lys1114 (Gln1113Arg)
Accession # AAH45606.1

Specificity

Detects human Nidogen‑1/Entactin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Nidogen-1/Entactin Antibody (302117)

Detection of Nidogen‑1/Entactin in Human Heart.

Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Nidogen-1/Entactin antibody in Human Chondrocytes by Immunocytochemistry (ICC).

Nidogen‑1/Entactin in Human Chondrocytes.

Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human Nidogen-1 / Entactin ELISA Standard Curve

Human Nidogen-1 / Entactin ELISA Standard Curve

Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND) was serially diluted and captured by Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Nidogen-1/Entactin by Immunohistochemistry

Detection of Nidogen-1/Entactin by Immunohistochemistry

Zebularine improves wound healing and limbal stem cell phenotype in diabetic organ-cultured corneas via Wnt-5a. (a) Wound healing time was significantly decreased in heptanol-wounded diabetic corneas treated with zebularine (20 µmol/l) vs DMSO (control) (n=4). (b–g) Immunostaining (n=3) showed that zebularine decreased DNMT1 (b) but increased Wnt-5a (c) levels in healed corneas. Zebularine also increased limbal levels of putative stem cell markers, K15 (d) and K17 (e), as well as diabetic markers integrin alpha 3 beta 1 (f) and nidogen-1 (g). Values are mean ± SEM. **p<0.01 vs control (paired t test). Scale bar, 20 µm. e, epithelium; s, stroma Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s00125-023-05960-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Nidogen-1/Entactin Antibody (302117)

Application
Recommended Usage

Immunocytochemistry

3-25 μg/mL                    Immersion fixed human mesenchymal stem cells differentiated into chondrocytes

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

1 µg/mL
Sample: Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND)

Human Nidogen-1/Entactin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: (Catalog # BAF2570)
  • Standard: Recombinant Human Nidogen-1/Entactin Protein, CF (Catalog # 2570-ND)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4 using MAB2570 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.

References

  1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
  5. Nagayoshi, T. et al. (1989) DNA 8:581. 
  6. Zimmerman, K. et al. (1995) Genomics 27:245. 
  7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
  8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
  9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
  10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
  11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
  12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.

Alternate Names

Entactin-1, NID1, Nidogen1

Entrez Gene IDs

4811 (Human)

Gene Symbol

NID1

UniProt

AAH45606.1

Additional Nidogen-1/Entactin Products

Product Documents for Human Nidogen-1/Entactin Antibody (302117)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Nidogen-1/Entactin Antibody (302117)

For research use only

Citations for Human Nidogen-1/Entactin Antibody (302117)

Customer Reviews for Human Nidogen-1/Entactin Antibody (302117) (7)

4 out of 5
7 Customer Ratings
5 Stars
29%
4 Stars
43%
3 Stars
29%
2 Stars
0%
1 Stars
0%

Have you used Human Nidogen-1/Entactin Antibody (302117)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 7 reviews Showing All
Filter By:
Clear Filters X
  • Human Nidogen-1/Entactin Antibody
    Name: Ruchi Shah
    Application: Immunohistochemistry
    Sample Tested: corneal tissue
    Species: Human
    Verified Customer | Posted 03/21/2023
    1% formalin fixed
    Human Nidogen-1/Entactin Antibody (302117) MAB2570
  • Human Nidogen-1/Entactin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 11/01/2021
    Human Nidogen-1/Entactin Antibody (302117) MAB2570
  • Human Nidogen-1/Entactin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Nidogen-1/Entactin Antibody (302117) MAB2570
  • Human Nidogen-1/Entactin Antibody
    Name: Pinyuan Tian
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Differentiated embryonic stem cells
    Species: Human
    Verified Customer | Posted 12/02/2020
    Staining for basement membrane
    Human Nidogen-1/Entactin Antibody (302117) MAB2570
  • Human Nidogen-1/Entactin Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Nidogen-1/Entactin Antibody (302117) MAB2570
  • Human Nidogen-1/Entactin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human Nidogen-1/Entactin Antibody
    Name: Ed Lo
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

There are no reviews that match your criteria.

Showing  1 - 5 of 7 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies