Human NIK/MAP3K14 Antibody (603917)

R&D Systems | Catalog # MAB6888

R&D Systems
Discontinued Product
MAB6888 has been discontinued. View all NIK/MAP3K14 products.

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 603917
View all NIK/MAP3K14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NIK/MAP3K14
Glu769-Pro947
Accession # Q99558

Specificity

Detects human NIK/MAP3K14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NIK/MAP3K14 Antibody (603917)

NIK/MAP3K14 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

NIK/MAP3K14 in Human Pancreas.

NIK/MAP3K14 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human NIK/MAP3K14 Monoclonal Antibody (Catalog # MAB6888) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human NIK/MAP3K14 Antibody (603917)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 1 review rated 4 using MAB6888 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NIK/MAP3K14

NIK (NF kappa B inducing kinase), also called MAP3K14, is a widely expressed 947 amino acid (aa), ~100 kDa cytoplasmic protein of the MAP kinase family. NIK participates in a non-canonical NFkB signaling cascade in which its activity and expression are elevated by TNF and related ligands. NIK activates IKKa/b, which releases NFkB subunits for translocation to the nucleus. Within the region used as an immunogen, human NIK shares 94% and 95% aa sequence identity with mouse and rat NIK, respectively.

Long Name

NF-kappa-beta Inducing Kinase

Alternate Names

FTDCR1B, HSNIK, MAP3K14

Entrez Gene IDs

9020 (Human); 53859 (Mouse); 360640 (Rat)

Gene Symbol

MAP3K14

UniProt

Q99558

Additional NIK/MAP3K14 Products

Product Documents for Human NIK/MAP3K14 Antibody (603917)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NIK/MAP3K14 Antibody (603917)

For research use only

Customer Reviews for Human NIK/MAP3K14 Antibody (603917) (1)

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  • Human NIK/MAP3K14 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 07/16/2020
    Human NIK/MAP3K14 Antibody (603917) MAB6888

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Protocols

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