Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2.
Human Ninjurin-1 Antibody (758943)
R&D Systems | Catalog # MAB5105
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Key Product Details
Species Reactivity
Validated:
Cited:
Applications
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Label
Antibody Source
Product Specifications
Immunogen
Asp2-Val81
Accession # Q92982
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Ninjurin-1 Antibody (758943)
Detection of Human Ninjurin‑1 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ninjurin-1 Monoclonal Antibody (Catalog # MAB5105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ninjurin-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Ninjurin-1 Antibody (758943)
Western Blot
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Ninjurin-1
Alternate Names
Gene Symbol
UniProt
Additional Ninjurin-1 Products
Product Documents for Human Ninjurin-1 Antibody (758943)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Ninjurin-1 Antibody (758943)
For research use only
Related Research Areas
Citations for Human Ninjurin-1 Antibody (758943)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars