Human NKG2C/CD159c Antibody (134591)

R&D Systems | Catalog # MAB138

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Neutralization, Flow Cytometry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 134591
View all NKG2C/CD159c Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94

Specificity

Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NKG2C/CD159c Antibody (134591)

Detection of NKG2C/CD159c in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (MAB138) or (B) Mouse IgG1 Isotype Control (MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry

Phenotypic characterization of NK cells from HIV-infected individuals with different levels of adaptation to HCMV infection. To identify the NK cell population, (A) PBMC were selected and dead cells excluded. NK cells were identified by expression of CD56 in the absence of CD3 and further characterized for percentage expressing NKG2C, CD57, Tim-3, and FcR gamma in a representative plot. Summary plots depict the percentage of total NK cells that are positive for (B) NKG2C or (C) FcR gamma. Individual donors were grouped based on HCMV serostatus (HCMVneg) and fraction of NK cells expressing NKG2C within the HCMVpos donors (NKG2Clo or NKG2Chi). Correlation between NK cell NKG2C and FcR gamma expression was assessed in (D) with Spearman correlation coefficient (r) calculated and the probability of a significant correlation (p) shown on the graph. Error bars in (B) for the HCMVneg group represent median with IQR, while all others represent mean ± SD. Groups were compared using Mann–Whitney U-tests. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30483249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human NKG2C/CD159c Antibody (134591)

Application
Recommended Usage

CyTOF-reported

Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human PBMC

Reviewed Applications

Read 1 review rated 4 using MAB138 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Long Name

Natural Killer G2C

Alternate Names

CD159c, KLRC2, NKG2-C

Entrez Gene IDs

3822 (Human); 16642 (Mouse)

Gene Symbol

KLRC2

Additional NKG2C/CD159c Products

Product Documents for Human NKG2C/CD159c Antibody (134591)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NKG2C/CD159c Antibody (134591)

For research use only

Citations for Human NKG2C/CD159c Antibody (134591)

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  • Human NKG2C/CD159c Antibody
    Name: Stephanie Weiland
    Application: ELISA
    Sample Tested: AML-193 human acute monocytic leukemia cell line
    Species: B. subtilis
    Verified Customer | Posted 02/22/2016

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