Human NLRP1/NALP1 Antibody (447916)

R&D Systems | Catalog # MAB6788

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 447916
View all NLRP1/NALP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NLRP1/NALP1
Gly1331-Leu1429
Accession # Q9C000

Specificity

Detects human NLRP1/NALP1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NLRP1/NALP1 Antibody (447916)

NLRP1/NALP1 antibody in Human Stomach Cancer Tissue by Immunohistochemistry (IHC-P).

NLRP1/NALP1 in Human Stomach Cancer Tissue.

NLRP1/NALP1 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Mouse Anti-Human NLRP1/NALP1 Monoclonal Antibody (Catalog # MAB6788) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human NLRP1/NALP1 Antibody (447916)

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB6788 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NLRP1/NALP1

NALP1 (NAcht, Leucine-rich repeat and PYD domain containing protein 1; also NAC, CARD7, DEFCAP and CLR17.1) is a 160-170 kDa member of the NLRP family of molecules. It is expressed in dendritic cells, neutrophils, T and B cells, alveolar macrophages, permatogonia, neurons, plus intestinal columnar epithelium, and is found in both cytoplasm and nucleus. NALP1 promotes apoptosis plus IL-1 beta and IL-18 maturation by activating caspase-1 and -5. It does so by forming a 700 kDa inflammasome comprised of NALP1, ASC plus caspase-1 and -5. Bacterial wall peptidoglycan binds to NALP1, promoting ATP binding, NALP1 oligomerization, and caspase activation. Human NALP1 is 1473 amino acids (aa) in length. It contains an N-terminal DAPIN domain (aa 1-92), a NACHT domain (aa 328-637), seven consecutive LRRs (aa 704-1236) and one CARD region (aa 1374-1463). Alternate splice forms exist that range in size from 70 kDa-150 kDa. Either individually, or in combination, there can be a 19 aa substitution for either aa 1353-1473 or aa 1368-1472, a deletion of aa 91-260, 958-987 or 1262-1305, and a 43 aa insert after Leu785. Over aa 1-323, human NALP1 shares less that 20% aa identity with mouse NALP1.

Long Name

NLR Family, Pyrin Domain Containing 1

Alternate Names

CARD7, CLR17.1, DEFCAP, NALP1, PP1044, SLEV1, VAMAS1

Entrez Gene IDs

22861 (Human); 195046 (Mouse); 360557 (Rat)

Gene Symbol

NLRP1

UniProt

Q9C000 (Human)

Additional NLRP1/NALP1 Products

Product Documents for Human NLRP1/NALP1 Antibody (447916)

Certificate of Analysis

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Product Specific Notices for Human NLRP1/NALP1 Antibody (447916)

For research use only

Citations for Human NLRP1/NALP1 Antibody (447916)

Customer Reviews for Human NLRP1/NALP1 Antibody (447916) (1)

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  • Human NLRP1/NALP1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Human
    Verified Customer | Posted 09/10/2022
    Human NLRP1/NALP1 Antibody (447916) MAB6788

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Protocols

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Associated Pathways

NOD-like Receptor Signaling Pathways
NOD-like Receptor Signaling Pathway Thumbnail