Human NLRP2/NALP2 Antibody

R&D Systems | Catalog # AF4684

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all NLRP2/NALP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02

Specificity

Detects human NLRP2/NALP2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human NLRP2/NALP2 Antibody

Detection of Human NLRP2/NALP2 antibody by Western Blot.

Detection of Human NLRP2/NALP2 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NLRP2/NALP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4684) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human NLRP2/NALP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NLRP2/NALP2

NALP2 (NAcht Leucine-rich repeat Protein 2, also known as NLRP2 and PAN1) is a cytosolic, 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF-alpha production in response to LPS; via procaspase-1, it promotes proIL-1 beta cleavage and release. Human NALP2 is 1062 amino acids (aa) in length. It contains an N-terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa’s 109‑132, 133‑154 and 847‑1062, respectively; a fourth shows a 38 aa substitution for aa’s 1‑903. No close rodent counterparts have been reported.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

NALP2, NBS1, PAN1, PYPAF2

Entrez Gene IDs

55655 (Human); 232827 (Mouse)

Gene Symbol

NLRP2

UniProt

Q9NX02

Additional NLRP2/NALP2 Products

Product Documents for Human NLRP2/NALP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NLRP2/NALP2 Antibody

For research use only

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