Human Osterix/Sp7 Antibody (764704)

R&D Systems | Catalog # MAB7547

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 764704
View all Osterix/Sp7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2

Specificity

Detects human Osterix/Sp7 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Osterix/Sp7 Antibody (764704)

Detection of Human Osterix/Sp7 antibody by Western Blot.

Detection of Human Osterix/Sp7 by Western Blot.

Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Osterix/Sp7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Osterix/Sp7 antibody in Saos-2 Human Cell Line by Immunocytochemistry (ICC).

Osterix/Sp7 in Saos‑2 Human Cell Line.

Osterix/Sp7 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of hnjgl2vqc1i12svqq0f5.jpg by Western Blot

Detection of hnjgl2vqc1i12svqq0f5.jpg by Western Blot

The expression of Runx2 and SP7 in Ad-MSCs varies depending on the donor site. Ad-MSCs derived from adipose tissue of the knee, limb, abdomen, thigh, and hip (N = 4 each) were osteogenically differentiated for 14 days. The relative expression levels of (a) Runx2 and (b) SP7 were determined by semi-quantitative RT-PCR. The PCR products were loaded twice (n = 2) to minimize the loading errors. The signal intensities were quantified with the help of the ImageJ software; (c) Representative images for the RT-PCR products from the pooled samples; (d) Representative images for the Western blot signals obtained from the pooled samples; * p < 0.05 and ** p < 0.01 as indicated. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/19/7/1868), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Osterix/Sp7 Antibody (764704)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Saos‑2 human osteosarcoma cell line

Western Blot

1 µg/mL
Sample: Saos‑2 human osteosarcoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB7547 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osterix/Sp7

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Long Name

Sp7 Transcription Factor

Alternate Names

OSX, Sp7

Entrez Gene IDs

121340 (Human); 170574 (Mouse); 300260 (Rat)

Gene Symbol

SP7

UniProt

Q8TDD2 (Human)

Additional Osterix/Sp7 Products

Product Documents for Human Osterix/Sp7 Antibody (764704)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Osterix/Sp7 Antibody (764704)

For research use only

Citations for Human Osterix/Sp7 Antibody (764704)

Customer Reviews for Human Osterix/Sp7 Antibody (764704) (1)

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  • Human Osterix/Sp7 Antibody
    Name: Michela Rossi
    Application: Western Blot
    Sample Tested: Saos2 Cells
    Species: Human
    Verified Customer | Posted 05/25/2020
    Saos2 cells transfected with different expression of osterix
    Human Osterix/Sp7 Antibody (764704) MAB7547

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