Human PAK2 Antibody (677411)

Detection of Human, Mouse, and Rat PAK2 by Western Blot.

Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PAK2 Monoclonal Antibody (Catalog # MAB6849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of erw2itdfglz6sbdcfwsn.jpg by Western Blot

ADAM15- and N-cadherin (NCAD) dependent activation of PAK2 upon mechanical strain. (A–D) Immunoblots for pPAK2. (A) of RASFs seeded at different cell densities and mechanically strained for 30 and 60 min. (B) from strained RASFs with PAK2 silencing (si) and treated with a negative control siRNA (neg). (C, D) immunoblots for pPAK2, ADAM15 and NCAD from RASFs treated with ADAM15 and NCAD siRNA; right panels, showing the mean ± SD of densitometric analysis of 6 different RASF cell lines. ***p < 0.0005, Student’s t-test. GAPDH served as a loading control. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-025-94012-2), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PAK2 by Immunocytochemistry/ Immunofluorescence

Mechano-induced recruitment of PAK2 together with Nck to the cell membrane. (A, B) RASFs grown on Bioflex plates and either unstimulated or strained for 30 min were fluorescently stained for (A) NCAD and Nck and (B) NCAD and PAK2. Adjacent to images: quantification of the pixel density of NCAD /Nck or NCAD /PAK2 along the white arrow. (C) double staining of NCAD and PAK2 in RASFs with prior silencing of Nck, right, pixel density along arrow. Cell nuclei were counterstained with DAPI. Objective 40x, scale bar = 10 μm. (D) immunoblots for PAK2, pPAK2, Nck and NCAD. RASFs pre-treated with Nck siRNA (I) and a nonsilencing siRNA (N) were mechanically strained for 15 and 30 min, the cell surface biotinylated with non-membrane-permeable biotin reagent and purified on streptavidin-conjugated magnetic beads. Contr: non biotinylated cell lysates enriched on streptavidin beads served as background control. TL = total lysates from 30 min time point. GAPDH was used to control the purity of the plasma membrane fractions. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-025-94012-2), licensed under a CC-BY license. Not internally tested by R&D Systems.