Human Pancreatic Polypeptide/PP Antibody (548416)

R&D Systems | Catalog # MAB62971

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 548416
View all Pancreatic Polypeptide/PP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pancreatic Polypeptide/PP
Ala30-Leu95
Accession # P01298

Specificity

Detects human Pancreatic Polypeptide/PP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Pancreatic Polypeptide/PP Antibody (548416)

Pancreatic Polypeptide/PP antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Pancreatic Polypeptide/PP in Human Pancreas.

Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Pancreatic Polypeptide/ PP Monoclonal Antibody (Catalog # MAB62971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Pancreatic Polypeptide/PP Antibody (548416)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 1 review rated 5 using MAB62971 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pancreatic Polypeptide/PP

Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence (1). PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake (2). The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

References

  1. Leiter A, et al. (1984) J. Biol. Chem. 259:14702.
  2. Asakawa, A et al. (2003) Gastroenterology 124:1325.

Alternate Names

INN, Obinepitide, PNP, PP, PPY

Entrez Gene IDs

5539 (Human)

Gene Symbol

PPY

UniProt

P01298

Additional Pancreatic Polypeptide/PP Products

Product Documents for Human Pancreatic Polypeptide/PP Antibody (548416)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pancreatic Polypeptide/PP Antibody (548416)

For research use only

Related Research Areas

Nutrition and Appetite
Obesity

Citations for Human Pancreatic Polypeptide/PP Antibody (548416)

Customer Reviews for Human Pancreatic Polypeptide/PP Antibody (548416) (1)

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  • Human Pancreatic Polypeptide/PP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult pancreas
    Species: Human
    Verified Customer | Posted 02/26/2022
    Human Pancreatic Polypeptide/PP Antibody (548416) MAB62971

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