Human Pannexin-3 Antibody (788905)

R&D Systems | Catalog # MAB8169

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 788905
View all Pannexin-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pannexin-3
Tyr289-Thr384
Accession # Q96QZ0

Specificity

Detects human Pannexin-3 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pannexin-3 Antibody (788905)

Detection of Human Pannexin-3 antibody by Western Blot.

Detection of Human Pannexin-3 by Western Blot.

Western blot shows lysates of SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pannexin-3 Monoclonal Antibody (Catalog # MAB8169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pannexin-3 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Pannexin-3 Antibody (788905)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pannexin-3

Pannexin-3 (PANX3) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed in skin, cochlea, and developing hard tissues including cartilage and bone. In osteoblasts, it forms Ca2+ channels in the endoplasmic reticulum and promotes osteogenic differentiation. It also forms gap junction channels that pass Ca2+ between cells, and ATP-releasing hemichannels in the plasma membrane. Within aa 289-384 (in the C-terminal cytoplasmic domain), human Pannexin-3 shares 95% and 99% aa sequence identity with mouse and rat Pannexin-3, respectively.

Alternate Names

Pannexin3, PANX3, PX3

Entrez Gene IDs

116337 (Human); 208098 (Mouse); 315567 (Rat)

Gene Symbol

PANX3

UniProt

Q96QZ0

Additional Pannexin-3 Products

Product Documents for Human Pannexin-3 Antibody (788905)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pannexin-3 Antibody (788905)

For research use only

Related Research Areas

Ion Channels and Regulators

Citations for Human Pannexin-3 Antibody (788905)

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