Human PBEF/Visfatin Antibody (882108)

R&D Systems | Catalog # MAB43351

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 882108
View all PBEF/Visfatin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PBEF/Visfatin
Pro27-His491
Accession # P43490

Specificity

Detects human PBEF/Visfatin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PBEF/Visfatin Antibody (882108)

Human PBEF/Visfatin Antibody in ELISA Standard Curve.

Human PBEF/Visfatin ELISA Standard Curve.

Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human PBEF/Visfatin Antibody (882108)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PBEF/Visfatin DuoSet ELISA Kit (Catalog # DY4335-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

P43490

Additional PBEF/Visfatin Products

Product Documents for Human PBEF/Visfatin Antibody (882108)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PBEF/Visfatin Antibody (882108)

For research use only

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways
Adipokines & Insulin Signaling Pathway Thumbnail