Human phospho-CREB (S133) Antibody (702710)

R&D Systems | Catalog # MAB6906

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 702710
View all CREB Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human CREB S133 site 

Specificity

Detects human CREB when phosphorylated at S133.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human phospho-CREB (S133) Antibody (702710)

Detection of Human Phospho-CREB (S133) antibody by Western Blot.

Detection of Human Phospho-CREB (S133) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-CREB (S133) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Phospho-CREB (S133) in HeLa Human Cell Line.

CREB phosphorylated at A133 was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA, using Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-CREB (S133) Antibody (702710)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Hela human cervical epithelial carcinoma cells stimulated with PMA

Western Blot

0.5 µg/mL
Sample: Hela human cervical epithelial carcinoma cells and HEK293 human embryonic kidney cells stimulated with ultraviolet light (UV).

Reviewed Applications

Read 4 reviews rated 4 using MAB6906 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

Additional CREB Products

Product Documents for Human phospho-CREB (S133) Antibody (702710)

Certificate of Analysis

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Product Specific Notices for Human phospho-CREB (S133) Antibody (702710)

For research use only

Citations for Human phospho-CREB (S133) Antibody (702710)

Customer Reviews for Human phospho-CREB (S133) Antibody (702710) (4)

4 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
25%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human Phospho-CREB (S133) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neurons
    Species: Human
    Verified Customer | Posted 11/29/2021
    Human phospho-CREB (S133) Antibody (702710) MAB6906
  • Human Phospho-CREB (S133) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 04/16/2019
  • Human Phospho-CREB (S133) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 01/29/2019
    Human phospho-CREB (S133) Antibody (702710) MAB6906
  • Human Phospho-CREB (S133) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human fibroblast
    Species: Human
    Verified Customer | Posted 08/23/2018
    Human phospho-CREB (S133) Antibody (702710) MAB6906

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Protocols

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