Human phospho-PDGF R beta (Y751) Antibody

R&D Systems | Catalog # AF1767

R&D Systems
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

TaqMan Protein Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PDGF R beta Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human PDGF R beta Y751 site

Specificity

Detects human PDGF R beta when phosphorylated at Y751 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human phospho-PDGF R beta (Y751) Antibody

Detection of Human Phospho-PDGF R beta (Y751) antibody by Western Blot.

Detection of Human Phospho-PDGF R beta (Y751) by Western Blot.

Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-PDGF R beta (Y751) antibody by Simple WesternTM.

Detection of Human Phospho-PDGF R beta (Y751) by Simple WesternTM.

Simple Western lane view shows lysates of CCD-1070Sk human foreskin fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 274 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Human phospho-PDGF R beta (Y751) Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: CCD‑1070Sk human foreskin fibroblast cell line treated with Recombinant Human PDGF‑AA (Catalog # 221-AA)

Western Blot

0.5 µg/mL
Sample: Human foreskin fibroblast treated with Recombinant Human PDGF‑BB (Catalog # 220-BB

Reviewed Applications

Read 1 review rated 5 using AF1767 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin‑like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

References

  1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

5159 (Human); 18596 (Mouse); 24629 (Rat)

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for Human phospho-PDGF R beta (Y751) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-PDGF R beta (Y751) Antibody

For research use only

Citations for Human phospho-PDGF R beta (Y751) Antibody

Customer Reviews for Human phospho-PDGF R beta (Y751) Antibody (1)

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  • Human Phospho-PDGF R beta (Y751) Antibody
    Name: Alina Filatova
    Application: Western Blot
    Sample Tested: G55TL human glioblastoma cell line
    Species: Human
    Verified Customer | Posted 09/29/2017
    I used this antibody for Western blot in 1:500 dilution; detection with ECL. A strong signal could be detected upon PDGF stimulation of the cells.
    Human phospho-PDGF R beta (Y751) Antibody AF1767

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