Human phospho-TOR (S2448) Antibody (834115)

Detection of Human Phospho-TOR (S2448) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-TOR (S2448) by Flow Cytometry

Multi-modal characterization of minute and hour signal transduction(A) Violin plot of the factor 1 value as a function of the anti-immunoglobulin antibody (aIg) treatment duration. To reflect the biological intuition on increased phosphorylation upon stimulation, the y axis of factor 1 is flipped.(B) Protein loading contributing to factor 1, ranked according to loading value. Proteins with loading value less than −0.25 are annotated in the graphs; colors highlight gene sets related to BCR signaling (blue) or JAK1 (red).(C) BCR signaling pathway with phosphoproteins colored by MOFA+ factor 1 loading value.(D) Scaled and normalized QuRIE-seq counts of p-CD79a, p-SYK, and p-JAK1 in BJAB cells upon stimulation with aIg.(E) Violin plot of the factor 3 value as a function of the aIg treatment duration.(F) Protein (left) and gene (right) loading contributing to factor 3. Proteins and genes with loading value >0.25 are indicated in the graphs. Blue color highlights proteins or genes related to the indicated biological processes.(G) Top 10 gene ontology (GO) biological process terms enriched in factor 3 genes with positive loading values.(H) Scaled and normalized QuRIE-seq counts of NEAT1, NPM1, and BTF3 in BJAB cells upon stimulation with aIg. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35474668), licensed under a CC-BY license. Not internally tested by R&D Systems.