Human PNR/NR2E3 Antibody (H7223)

Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence

Coexpression of Rxrg and Nr2e3 in the embryonic mouse retina. (A-H) E14.5 mouse retinas electroporated with either the cNr2e3Enh2 or cNr2e3Enh3 plasmid, cultured ex vivo for 2 days, and processed for immunofluorescence confocal imaging of EGFP (green, chicken antibody), Nr2e3 (red, mouse antibody), Rxrg (white, rabbit antibody), and DAPI. Panels in A-H represent maximum projections of z-stacks with the depicted channel shown at the top of the column. The merge column has EGFP, Nr2e3, and Rxrg signals. (A’-H’) Magnified single z-plane images with the signals for (A’,E’) EGFP, (B’,F’) Nr2e3, (C’,G’) Rxrg, and (D’,H’) DAPI. White arrows point to GFP+ cells that also express Nr2e3 and Rxrg. Yellow arrows point to GFP+ cells that express Rxrg, but not Nr2e3. (I) A graph of the average percentage of GFP+ cells when driven by the cNr2e3Enh2 or cNr2e3Enh3 elements that express Nr2e3, Rxrg, or both Nr2e3 and Rxrg. (J-L) Maximum projection of a z-stack image of a E17.5 mouse retina processed for immunofluorescent detection of Nr2e3 (J, green) and Rxrg (K, red) or both (L, Merge). (J’-L’) Magnified single z-plane images of the same area visualized for signals for Nr2e3 (J’), Rxrg (K’), or DAPI (L’). (M) A graph of the average percentage of Nr2e3, Rxrg double-positive (D.P.) cells out of the total Nr2e3+ population (left bar) or the total Rxrg+ population (right bar). In both graphs N ≥ 3 biological replicates. Error bars represent standard error of the mean. All images are oriented with the scleral side of the retina at the top of the image. Scale bar in A represents 20 μm and applies to A-L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30466480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence

Coexpression of Rxrg and Nr2e3 in the embryonic mouse retina. (A-H) E14.5 mouse retinas electroporated with either the cNr2e3Enh2 or cNr2e3Enh3 plasmid, cultured ex vivo for 2 days, and processed for immunofluorescence confocal imaging of EGFP (green, chicken antibody), Nr2e3 (red, mouse antibody), Rxrg (white, rabbit antibody), and DAPI. Panels in A-H represent maximum projections of z-stacks with the depicted channel shown at the top of the column. The merge column has EGFP, Nr2e3, and Rxrg signals. (A’-H’) Magnified single z-plane images with the signals for (A’,E’) EGFP, (B’,F’) Nr2e3, (C’,G’) Rxrg, and (D’,H’) DAPI. White arrows point to GFP+ cells that also express Nr2e3 and Rxrg. Yellow arrows point to GFP+ cells that express Rxrg, but not Nr2e3. (I) A graph of the average percentage of GFP+ cells when driven by the cNr2e3Enh2 or cNr2e3Enh3 elements that express Nr2e3, Rxrg, or both Nr2e3 and Rxrg. (J-L) Maximum projection of a z-stack image of a E17.5 mouse retina processed for immunofluorescent detection of Nr2e3 (J, green) and Rxrg (K, red) or both (L, Merge). (J’-L’) Magnified single z-plane images of the same area visualized for signals for Nr2e3 (J’), Rxrg (K’), or DAPI (L’). (M) A graph of the average percentage of Nr2e3, Rxrg double-positive (D.P.) cells out of the total Nr2e3+ population (left bar) or the total Rxrg+ population (right bar). In both graphs N ≥ 3 biological replicates. Error bars represent standard error of the mean. All images are oriented with the scleral side of the retina at the top of the image. Scale bar in A represents 20 μm and applies to A-L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30466480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PNR/NR2E3 by Immunohistochemistry

NR2E3 and GNAT2 co-expression in photoreceptor precursors.(A) Composite images of combined RXR gamma and NR2E3 immunofluorescence staining and GNAT2 and NR2E3 RNA FISH in boxed regions 2–13 of Figure 7A. Scale bar = 50 µm. (B) Enlarged images of boxed regions in Figures 2, 3, 5 and 8 in panel A, as indicated at upper left of images in column 1. Dotted yellow lines demarcate the outermost and middle (sub-outermost) NBL cells analyzed separately in Figure 7C and D. White arrows = GNAT2 puncta in boxed regions of interest. Green arrows = NR2E3 puncta. Boxed regions in column 3 (GNAT2 RNA plus NR2E3 protein) and column 4 (NR2E3 RNA) are enlarged in column 6 (GNAT2 RNA plus NR2E3 RNA) and illustrate (region 2) initial expression of GNAT2 RNA without NR2E3 RNA in outermost NBL nascent cones; (region 3) initial co-expression of GNAT2 and NR2E3 RNA in early outermost NBL cones; (region 5) initial expression of NR2E3 RNA with or without co-expressed NR2E3 protein; and (region 8) co-expression of high GNAT2 and low NR2E3 RNA without NR2E3 protein in RXR gamma hi outermost NBL cones. Scale bars in columns 2 and 6 = 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40767624), licensed under a CC-BY license. Not internally tested by R&D Systems.