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VEGF 165 Antibody [Biotin]

R&D Systems | Catalog # BAF293

R&D Systems
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, Array Development, Bioassay, ELISA Detection, ELISA Development, ELISA Development (Detection), ELISA Microarray Development, Proximity Ligation Assay, Functional Assay, Quantum Dot, TaqMan Protein Assay

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165 (R&D Systems, Catalog # 293-VE)
Ala27-Arg191
Accession # AAV38412

Specificity

Detects human VEGF

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VEGF 165 Antibody [Biotin]

Detection of Rat VEGF by Western Blot

Detection of Rat VEGF by Western Blot

Protein levels of VEGF (A), KDR (VEGF receptor 2, B), Angiopoietin 1 (C) and Tie2 (D) from retinal endothelial cells cultured in normoxia or hypoxia only and cells in each condition treated with Compound 49b. Hypoxia increased protein levels of all proteins, which were significantly reduced after treatment with Compound 49b. Data is mean ± SEM. *P<0.05 vs. normoxia. #P<0.05 vs. hypoxia. N = 4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27439004), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human VEGF ELISA Standard Curve

Human VEGF ELISA Standard Curve

Recombinant Human VEGF 165 (Catalog # 293-VE) was serially diluted and captured by Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGF 165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human VEGF DuoSet ELISA

Human VEGF DuoSet ELISA

Recombinant Human VEGF 165 (Catalog # 293-VE) was serially diluted and captured by Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGF 165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for VEGF 165 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human VEGF165 (Catalog # 293-VE)

Human/Primate VEGF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate VEGF Antibody (Catalog # MAB293)
  • Capture Reagent: Human/Primate VEGF Antibody (Catalog # MAB293R)
  • Standard: Recombinant Human VEGF 165 Protein (Catalog # 293-VE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF293 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

AAV38412

Additional VEGF Products

Product Documents for VEGF 165 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for VEGF 165 Antibody [Biotin]

For research use only

Citations for VEGF 165 Antibody [Biotin]

Customer Reviews for VEGF 165 Antibody [Biotin] (1)

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Customer Images

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  • Human/Primate VEGF165 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    This antibody was used as the detection in an ELISA targeting VEGF in human serum samples.
    VEGF 165 Antibody [Biotin] BAF293

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