Human Pro Coagulation Factor II/Thrombin Antibody (200710)

R&D Systems | Catalog # MAB1473

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 200710
View all Coagulation Factor II/Thrombin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pro Coagulation Factor II/Thrombin
Ala44-Glu622
Accession # NP_000497

Specificity

Detects human Pro Coagulation Factor II/Thrombin in direct ELISAs and Western blots. It recognizes only Pro Coagulation Factor II/Thrombin, not active Thrombin. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Factor X, rhKallikrein 1, 3, 5, and 11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pro Coagulation Factor II/Thrombin Antibody (200710)

Detection of Human Pro Coagulation Factor II/Thrombin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pro Coagulation Factor II/Thrombin Monoclonal Antibody (Catalog # MAB1473) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Pro Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Pro Coagulation Factor II/Thrombin Antibody (200710)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Coagulation Factor II/Thrombin (Catalog # 2196-SE), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor II/Thrombin

Pro-Coagulation Factor II, also called Prothrombin, is a plasma serine protease central to the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin. Pro-Coagulation Factor II is an inactive precursor of thrombin that can be activated by proteolytic removal of the pro region.

Alternate Names

F2, Thrombin

Entrez Gene IDs

2147 (Human); 14061 (Mouse)

Gene Symbol

F2

UniProt

NP_000497

Additional Coagulation Factor II/Thrombin Products

Product Documents for Human Pro Coagulation Factor II/Thrombin Antibody (200710)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro Coagulation Factor II/Thrombin Antibody (200710)

For research use only

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways
Blood Coagulation Signaling Pathway Thumbnail