Human Protocadherin-15 Antibody (740523)

R&D Systems | Catalog # MAB6729

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 740523
View all Protocadherin-15 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin-15
Tyr28-Ala1376
Accession # Q96QU1

Specificity

Detects human Protocadherin-15 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Protocadherin-15 Antibody (740523)

Protocadherin-15 antibody in YT Human Cell Line by Immunocytochemistry (ICC).

Protocadherin‑15 in YT Human Cell Line.

Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Mouse Anti-Human Protocadherin-15 Monoclonal Antibody (Catalog # MAB6729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Protocadherin-15 Antibody (740523)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed YT human leukemia natural killer‑like cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB6729 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin-15

Protocadherin-15 (PCDH-15) is a 250-270 kDa "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereocilia in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929 amino acids (aa) in length. It contains a 1350 aa extracellular domain (ECD) (aa 27-1376) plus a 558 aa cytoplasmic region. There are 11 cadherin domains in the ECD (aa 40-1259). Multiple splice forms are reported. One is a soluble 110 kDa form that shows a five aa substitution for aa 957-1955. Another contains a seven aa substitution for aa 1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa 27-1376 (the ECD), human PCDH-15 shares 95% aa sequence identity with mouse PCDH-15.

Alternate Names

CDHR15, DFNB23, PCDH15, Protocadherin15, USH1F

Entrez Gene IDs

65217 (Human); 11994 (Mouse); 690865 (Rat)

Gene Symbol

PCDH15

UniProt

Q96QU1 (Human)

Additional Protocadherin-15 Products

Product Documents for Human Protocadherin-15 Antibody (740523)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protocadherin-15 Antibody (740523)

For research use only

Related Research Areas

Cadherin Superfamily

Customer Reviews for Human Protocadherin-15 Antibody (740523) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Protocadherin-15 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: YT Human Cell Line
    Species: Human
    Verified Customer | Posted 11/03/2022
    Human Protocadherin-15 Antibody (740523) MAB6729
  • Human Protocadherin-15 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Protocadherin-15 Antibody (740523) MAB6729
  • Human Protocadherin-15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018

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