Human PTHLH/PTHrP Antibody (677939)

R&D Systems | Catalog # MAB6734

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 677939
View all PTHLH/PTHrP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTHLH/PTHrP
Ala37-Arg175
Accession # P12272

Specificity

Detects human PTHLH/PTHrP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PTHLH/PTHrP Antibody (677939)

PTHLH/PTHrP antibody in Human Colon by Immunohistochemistry (IHC-P).

PTHLH/PTHrP in Human Colon.

PTHLH/PTHrP was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human PTHLH/PTHrP Monoclonal Antibody (Catalog # MAB6734) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of PTHLH/PTHrP by Immunohistochemistry

Detection of PTHLH/PTHrP by Immunohistochemistry

Treatment with a clinically applicable FAK inhibitor blocks EZH2-induced breast cancer bone metastasis.a Representative bioluminescence images of and quantification of the fold change in BLI intensity in the right leg region in three subgroups of mice (n = 5 in each group) intratibially injected with MDA-MB-231 cells and given treatment with a vehicle, GSK126 (100 mg/kg/day, i.p. injection), or the FAKi VS-6063 (50 mg/kg, twice a day, oral gavage) beginning on day 18 of injection. The BLI signal was normalized according to the signal on the first day after injection. Data are presented as means ± S.D. t-test (two-sided). b, c Representative pictures of IHC staining and quantification of pS465/467-Smad2 and PTHLP expression in the bone metastasis samples obtained from the three subgroups of mice in a. Data are presented as means ± S.E.M. t-test (two-sided). n = 4 bone metastasis slices in vehicle subgroup; n = 5 bone metastasis slices in GSK126 subgroup; n = 3 bone metastasis slices in FAKi subgroup. Scale bars, 50 μm. d The Pearson r correlation for EZH2 RNA expression and bone metastasis-free survival years (bmfs Yr) in breast cancer patients (GSE2603 dataset). e The Pearson r correlation for EZH2 and PTHLH RNA mRNA expression in bone metastasis samples obtained from breast cancer patients (GSE14020 dataset). All P-values are indicated in the figures. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-022-30105-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PTHLH/PTHrP Antibody (677939)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Reviewed Applications

Read 1 review rated 3 using MAB6734 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTHLH/PTHrP

The parathyroid hormone-like hormone (PTHLH), also known as PTHrP, has been recognized in patients suffering from tumors associated with the common neoplastic syndrome humoral hypercalcemia of malignancy (HHM). The 18 kDa protein is a 177 amino acid (aa) precursor that belongs to the parathyroid hormone family. It contains a 24 aa signal sequence followed by a 10 aa propeptide (aa 25-34). The mature chain runs from aa 37-177. Of note, residues 108-129 constitute a nuclear localization signal (NLS) providing evidence that the protein not only acts as a secreted factor, but in an intracrine manner as well. A second isoform is missing aa 176-177, and a 34 aa substitution for aa 176-177 produces a third isoform. There is a strong homology displayed between PTHLH and human parathyroid hormone (PTH) among the first 13 aa. Studies show that the similarity in this area is 67% at the aa and nucleotide levels. Indeed, eight of the first 13 aa of PTHLH and PTH are identical. Human PTHLH and mouse PTHLH share 86% aa sequence identity.

Long Name

Parathyroid Hormone-like Hormone

Alternate Names

BDE2, HHM, Osteostatin, PTHRP

Entrez Gene IDs

5744 (Human); 19227 (Mouse); 24695 (Rat)

Gene Symbol

PTHLH

UniProt

P12272

Additional PTHLH/PTHrP Products

Product Documents for Human PTHLH/PTHrP Antibody (677939)

Certificate of Analysis

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Product Specific Notices for Human PTHLH/PTHrP Antibody (677939)

For research use only

Citations for Human PTHLH/PTHrP Antibody (677939)

Customer Reviews for Human PTHLH/PTHrP Antibody (677939) (1)

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  • Human PTHLH/PTHrP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: lung cancer cells
    Species: Human
    Verified Customer | Posted 09/23/2023
    We used this mouse-derived antibody to try western detection. 25ug was dissolved in 10ml TBST. In a variety of lung cancer cell lines, we detected some bands, but the signal was relatively weak. There were some differences between groups after long-term exposure. If you use this For WB experiments with antibodies, it is recommended to increase the detection concentration to about 1:500.
    Human PTHLH/PTHrP Antibody (677939) MAB6734

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