DYRK1A Antibody

R&D Systems | Catalog # AF5407

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Xenopus

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all DYRK1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DYRK1A
Arg438-Thr511
Accession # Q13627

Specificity

Detects human and rat DYRK1A in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for DYRK1A Antibody

DYRK1A antibody in Human Kidney by Immunohistochemistry (IHC-P).

DYRK1A in Human Kidney.

DYRK1A was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat samples.
Detection of Human/Rat DYRK1A antibody by Western Blot.

Detection of Human/Rat DYRK1A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DYRK1A at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human DYRK1A by Simple WesternTM.

Left: Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.1 mg/ml. A specific band was detected for DYRK1A at approximately 110 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the Jurkat human acute T cell leukemia cell line.
Detection of DYRK1A by Immunoprecipitation.

Detection of DYRK1A by Immunoprecipitation.

5637 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) pre-coupled to Dynabeads Protein G. Immunoprecipitated DYRK1A was detected in Western Blot with a rabbit anti-DYRK1A antibody used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Detection of Human DYRK1A antibody by Simple WesternTM.

Detection of Human DYRK1A by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DYRK1A at approximately 113 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for DYRK1A Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

2 µg/1 mg cell lysate
Sample: Cell lysate of human bladder carcinoma 5637 cell line

Simple Western

10-50 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYRK1A

DYRK1A (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 1A; also hMNB) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK1A is ubiquitously expressed and shows dual substrate specificity. Autophosphorylation on Tyr321 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include APP, Tau, STAT3, and Cyclin L2. Human DYRK1A is 763 amino acids (aa) in length. It contains two NLSs (aa 69‑105 and 109-150), a kinase catalytic domain (aa 159-479), and C-terminal poly-Ser and poly-His regions. DYRK1A activity has been implicated in neuronal development and Down syndrome.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 1A

Alternate Names

MNBH

Entrez Gene IDs

1859 (Human); 13548 (Mouse); 25255 (Rat)

Gene Symbol

DYRK1A

UniProt

Q13627

Additional DYRK1A Products

Product Documents for DYRK1A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for DYRK1A Antibody

For research use only

Related Research Areas

Intracellular Kinases
Notch Pathway

Citations for DYRK1A Antibody

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail