Lyn Antibody (338716)
R&D Systems | Catalog # MAB3206
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 338716
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Product Specifications
Immunogen
E. coli-derived recombinant human Lyn
Met1-Ile67
Accession # P07948
Met1-Ile67
Accession # P07948
Specificity
Detects human and rat Lyn.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Lyn Antibody (338716)
Detection of Human/Rat Lyn by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyn at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lyn in Raji Human Cell Line.
Lyn was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human Lyn by Simple WesternTM.
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyn at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Lyn Antibody (338716)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Simple Western
5 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line
Sample: Ramos human Burkitt's lymphoma cell line
Western Blot
0.2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Lyn
Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog
Alternate Names
Hck-2, JTK8, v-yes-1
Gene Symbol
LYN
UniProt
Additional Lyn Products
Product Documents for Lyn Antibody (338716)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Lyn Antibody (338716)
For research use only
Related Research Areas
Citations for Lyn Antibody (338716)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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