OV-6 Antibody (OV-6)

R&D Systems | Catalog # MAB2020

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat, Xenograft

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Cell Selection, MACS

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OV-6
View all OV-6 Primary Antibodies »

Product Specifications

Immunogen

Carcinogen-treated rat nodular hepatocytes

Specificity

Detects human and rat OV-6 antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for OV-6 Antibody (OV-6)

OV-6 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

OV‑6 in BG01V Human Embryonic Stem Cells.

OV-6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV-6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of OV-6 by Immunohistochemistry

Detection of OV-6 by Immunohistochemistry

Capsaicin reduced hepatic progenitor cells (HPCs) activation, and induced HPCs apoptosis in the liver of diethylnitrosamine (DEN)‐induced rats suffering from hepatocellular carcinoma. (A) Immunohistochemistry staining showed that the expression of HPCs markers, EpCAM, OV6, SOX9 as well as proliferation marker PCNA was decreased in the DEN+capsaicin group compared to the DEN group. (B) Tunnel staining showed a higher number of apoptotic cells in the DEN+capsaicin group than that in the DEN group. (C) Immunofluorescence co‐staining of Caspase‐3 and EpCAM suggested capsaicin‐induced HPCs apoptosis in DEN‐treated rat liver. Scale bars, 40 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35674129), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for OV-6 Antibody (OV-6)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

Parent, R. et al. (2004) Gastroenterology 126(4):1147.
He, Z.P. et al. (2003) Cell Prolif. 37(2):177.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cell bodies and BG01V human embryonic stem cells differentiated into hepatocytes

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OV-6

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

References

  1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
  2. Parent, R. et al. (2004) Gastroenterology 126:1147.
  3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
  4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
  5. He, Z.P. et al. (2003) Differentation 71:281.

Long Name

Oval Cell Marker

Alternate Names

OV6

Additional OV-6 Products

Product Documents for OV-6 Antibody (OV-6)

Certificate of Analysis

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Product Specific Notices for OV-6 Antibody (OV-6)

For research use only

Citations for OV-6 Antibody (OV-6)

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Protocols

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