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Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Syntaxin 5 isoform 2
Met55-Thr300
Accession # Q13190
Met55-Thr300
Accession # Q13190
Specificity
Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Syntaxin 5 Antibody
Detection of Human Syntaxin 5 by Western Blot.
Western blot shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Syntaxin 5 at approximately 35 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Syntaxin 5 in SH-SY5Y Human Cell Line.
Syntaxin 5 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Syntaxin 5 in Human Brain.
Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Syntaxin 5 in Rat Brain.
Syntaxin 5 was detected in immersion fixed frozen sections of rat brain using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 3 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human Syntaxin 5 by Simple WesternTM.
Simple Western lane view shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Syntaxin 5 at approximately 39 and 47 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Syntaxin 5 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and immersion fixed frozen sections of rat brain
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and immersion fixed frozen sections of rat brain
Simple Western
25 µg/mL
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Western Blot
0.5 µg/mL
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Sample: T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Syntaxin 5
Alternate Names
SED5, STX5
Gene Symbol
STX5
UniProt
Additional Syntaxin 5 Products
Product Documents for Syntaxin 5 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Syntaxin 5 Antibody
For research use only
Citations for Syntaxin 5 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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