Human RelA/NFkB p65 Antibody (465003)

Name: Human RelA/NFkB p65 Antibody (465003)
Brand: R&D Systems
SKU: MAB50781
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB50781

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 465003

View all RelA/NFkB p65 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RelA/NF kappa B p65
Met1-Thr254
Accession # NP_068810

Specificity

Detects human RelA/NF kappa B p65 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human RelA/NFkB p65 Antibody (465003)

Detection of RelA/NF kappa B p65 in Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was stained with Human RelA/NF kappa B p65 Monoclonal Antibody (Catalog # MAB50781, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human RelA/NFkB p65 Antibody (465003)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: RelA/NFkB p65

RelA p65 is a 65 kDa member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks amino acids (aa) 222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors.