Human Resistin Antibody (184320) [Biotin]

R&D Systems | Catalog # BAM1359

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 184320
View all Resistin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Resistin
Accession # Q9HD89

Specificity

Detects human Resistin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse RELM alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Resistin Antibody (184320) [Biotin]

Human Resistin ELISA Standard Curve

Human Resistin ELISA Standard Curve

Recombinant Human Resistin (Catalog # 1359-RN) was serially diluted and captured by Mouse Anti-Human Resistin Monoclonal Antibody (Catalog # MAB13591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Resistin Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Resistin Antibody (184320) [Biotin]

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Resistin
under non-reducing conditions only

Human Resistin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Resistin Antibody (Catalog # MAB13591)
  • Standard: Recombinant Human Resistin Protein, CF (Catalog # 1359-RN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAM1359 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Resistin

Resistin (resistance-to-insulin), also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis (1-4). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. The mature region shows an N-terminal alpha -helical tail (aa 23-44) and a C-terminal beta -sheet globular head (aa 47-108) (5-7). The Resistin molecule circulates as either a noncovalent trimer (minor form), or a disulfide-linked homohexamer (major form). Noncovalent trimers are generated when the alpha -helical segments self-associate to form a three-stranded coiled-coil structure. Covalent hexamers subsequently appear when the free Cys at position #26 is engaged by adjacent trimers. It is hypothesized that the hexamer is the inactive form of the molecule, and bioactivity is achieved at the target site by disulfide bond reduction (5). Although Resistin family molecules can noncovalently interact to form heterotrimers in vitro, there is no evidence to suggest this occurs in vivo with Resistin (8, 9). Mature human Resistin shares 56% and 54% aa identity with mouse and rat Resistin, respectively. Rat Resistin possesses an alternate start site at Met48; this Met is not found in the mouse molecule, however (10). Rodent resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium (6, 11, 12). Although the function of Resistin is unclear, it would seem to block insulin-stimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes (6, 13, 14). As such, it has been proposed to participate in diet-induced insulin-sensitivity. Diets high in fat promote an increase in overall adipocyte size. Hypertrophic adipocytes are known to secrete TNF-alpha which acts locally to block ACRP30 production. Since ACRP30 is an insulin-sensitizer, a drop in ACRP30 availability leads to insulin-insensitivity, which drives increased insulin production (a compensatory mechanism). High insulin induces Resistin secretion which now antagonizes insulin action, prompting more insulin production and more Resistin secretion (15).

References

  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. King, I.A. et al. (1993) Genomics 18:185.
  5. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
  6. King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.
  7. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
  8. Chidgey, M. et al. (2001) J. Cell Biol. 155:821.
  9. Khan, K. et al. (2006) Br. J. Cancer 95:1367.
  10. Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.
  11. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  12. Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Gene Symbol

RETN

UniProt

Q9HD89

Additional Resistin Products

Product Documents for Human Resistin Antibody (184320) [Biotin]

Certificate of Analysis

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Product Specific Notices for Human Resistin Antibody (184320) [Biotin]

For research use only

Related Research Areas

Glucose Homeostasis
Obesity

Citations for Human Resistin Antibody (184320) [Biotin]

Customer Reviews for Human Resistin Antibody (184320) [Biotin] (1)

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  • Human Resistin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/27/2017
    MAB7821 was used as the solid phase antibody, BAM1359 was used as the detection antibody. 1259-RN was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.
    Human Resistin Antibody (184320) [Biotin] BAM1359

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Associated Pathways

Adipokines & Insulin Signaling Pathways
Adipokines & Insulin Signaling Pathway Thumbnail