Human RIPK2/RIP2 Antibody (2527A)

R&D Systems | Catalog # MAB103872

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2527A
View all RIPK2/RIP2 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human RIPK2/RIP2
Met1-Met540
Accession # O43353

Specificity

Detects human RIPK2/RIP2 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human RIPK2/RIP2 Antibody (2527A)

Detection of Human RIPK2/RIP2 antibody by Western Blot.

Detection of Human RIPK2/RIP2 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103872) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human RIPK2/RIP2 Antibody (2527A)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RIPK2/RIP2

Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a  540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .

Long Name

Receptor Interacting Serine/Threonine Kinase 2

Alternate Names

CARD3, GIG30, RICK, RIP-2, RIP2

Entrez Gene IDs

8767 (Human); 192656 (Mouse)

Gene Symbol

RIPK2

UniProt

O43353

Additional RIPK2/RIP2 Products

Product Documents for Human RIPK2/RIP2 Antibody (2527A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RIPK2/RIP2 Antibody (2527A)

For research use only

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